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KMID : 0923620150150040206
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Immune Network
2015 Volume.15 No. 4 p.206 ~ p.211
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Involvement of Protein Kinase C-δ in Vascular Permeability in Acute Lung Injury
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:Ahn Jong-J.
:Park Soon E./:Jung Jong-P./:Lee Min-hyun/:Kwon Byung-Suk/:Cho Hong-R.
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Abstract
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Pulmonary edema is a major cause of mortality due to acute lung injury (ALI). The involvement of protein kinase C-δ (PKC-δ) in ALI has been a controversial topic. Here we investigated PKC-δ function in ALI using PKC-δ knockout (KO) mice and PKC inhibitors. Our results indicated that although the ability to produce proinflammatory mediators in response to LPS injury in PKC-δ KO mice was similar to that of control mice, they showed enhanced recruitment of neutrophils to the lung and more severe pulmonary edema. PKC-δ inhibition promoted barrier dysfunction in an endothelial cell layer in vitro, and administration of a PKC-δ-specific inhibitor significantly increased steady state vascular permeability. A neutrophil transmigration assay indicated that the PKC-δ inhibition increased neutrophil transmigration through an endothelial monolayer. This suggests that PKC-δ inhibition induces structural changes in endothelial cells, allowing extravasation of proteins and neutrophils.
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KEYWORD
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Acute lung injury, Protein kinase C-δ, Vascular permeability
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